Tomihara received his Ph.D. degree in 2006 from Sapporo Medical University, working on immunegene therapy by adenovirus vector. He then moved to Cancer Therapy and Research Center (CTRC) at The University of Texas Health Science Center at San Antonio(UTHSCSA) to work with Dr. Shin as a post-doctoral fellow. He obtained an assistant professor position in 2013 in the Department of Oral and Maxillofacial Surgery Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, where he started independent research on cancer immunology.
Background: Salivary duct carcinoma (SDC) is an uncommon neoplasm that most commonly occurs in major salivary glands, mainly the parotid gland. SDC is rarely found in the minor salivary glands of the oral cavity. Methods: Here we report an extremely rare case of sarcomatoid SDC originating in a minor salivary gland of the palate. The tumor was histologically characterized by the presence of both carcinomatous and sarcomatoid components. The patient presented with a painless mass in the right palate, which slowly increased in size over 20 years. Results: The clinical course of the present case suggests that the tumor most probably developed as a result of malignant transformation of a preexisting benign tumor of the palatal salivary gland.
Conclusions: We herein describe the clinical and histological features of this extremely rare case of sarcomatoid SDC with reference to the relevant literature.
Sulaiman Ali Alharbi has completed his Ph.D. at the age of 38 years from Sheffield University, England and has trained molecular techniques from Department of Molecular Biology and Biotechnology, Sheffield University. Presently he has been working as a Professor in Medical Bacteriology, Department of Botany and Microbiology and also he is a Vice Dean for Development and Quality, Deanship of Scientific Research, King Saud University, Riyadh. He has precise energetic and enthusiastic Microbiologist and has 15 years’ experience in both teaching and research activity. He has participated in several International, National conference, symposia, workshops and presented his research papers in several countries and recently he presented his novel research in Brazil. He has published more than 40 papers in reputed national, international and ISI journals and serving as an editorial board member of reputed journal and also has lifetime membership of microbiology associations in worldwide.
An antifungal protein synthesized by Bacillus cereus has been partially purified by the use of ammonium sulfate precipitation and Sephadex-G-200 column chromatography. The protein was produced from Bacillus cereus grown in potato dextrose broth medium (PDB) at 30ºC for 3 days at 100 rpm. The protein showed antagonistic effect against some fungi and yeasts. Crude extract from medium and semi-purified protein were tested in vitro against both fungi and yeasts using the disc diffusion method in order to detect the inhibitory effect of the protein. Zones of inhibition of the following diameter were found (mm) were Alternaria alternate (28), Rhodotorula glutinis (20), Fusarium sp. (16), Rhizopus sp. (15), Penicillium digitatum (13), Mucor sp. (13) and Aspergillus niger (10). The isolated protein was found to have a molecular weight of ~35kDa by sodium deodecyl sulfate-poly acrylamide gel electrophoresis. The data showed that the protein of Bacillus cereus has antifungal activity, a fact which points to the possibility of using it as a biocontrol agent against some fungi, findings which emphasize the potential role of B. cereus as an important biocontrol agent.
Teklil Biza Gizaw is working in Ethiopia Health and Nutrition Research institute as researcher and he is the coordinator of Bacteriology and Mycology research case team in the institute. He completed his graduate program in Clinical laboratory sciences at the age of 31 and published more than 5 original articles related to bacterial disease and drug resistance pattern in local journals. He has plenty of experience on conventional culture and drug sensitivity test. And also he is an executive member of Ethiopian medical laboratory association and providing different microbiology course for medical students.
Lower respiratory tract infection is an important cause of morbidity and mortality in all age groups. Each year 7 million people die due to direct consequences of acute and chronic respiratory diseases. Infectious lower respiratory disease might be caused by Fungal and bacterial agents. However, in various countries including Ethiopia, individuals who have chronic lower respiratory diseases are mostly suspected as having tuberculosis. Several literatures showed that the prevalence of smear negative pulmonary TB cases was increased in the previous two decades. The mortality rate of smear negative patients was greater than smear positive patients in HIV infected individuals.
Objectives: To determine the prevalence of bacterial and fungal agents on smear negative pulmonary tuberculosis registered patients in selected health facilities of Addis Ababa
Materials and Methods: A cross sectional study was conducted from November 2010 to May 2011. A total of 229 smear negative Pulmonary TB registered patients in selected health facilities in Addis Ababa were considered and sputum samples were collected from all of them. Common bacterial and fungal agents were detected using microscope and culture techniques. The finding of study was analyzed using descriptive statistic to determine their prevalence of bacterial and fungal agents, Moreover fisher exact & Chi-square tests were used to see association of agents & diseases. Magnitudes of associations were then measured by Binary regression analysis.
Result: Our study has suggests that there was no statistically associated with specific etiology identified in culture except weight loss and shortness of breath for Mycobacterium species. Similarly, chest x-ray features were not associated. Among the 229 study participants, 150 (65.5%) showed microbial infection. Infection with fungi, bacteria and Mycobacterium species were identified in 120 (52.4%), 48 (20.8%) and 31(13.5 %) respectively. Moreover,198 (86.5%) were negative for Mycobacterium species using culture techniques; and out of culture negative Tb cases, 102 (51.5%) and 40 (20.2%) study participants had fungal and bacterial infection respectively. From the isolated genus of fungi and bacteria, Aspergillus species and K.pneumoniae were the most frequent microbes respectively. Out of drugs were used for testing isolates, 59.8% was showed resistance by isolates and 28.2% of drugs were showed sensitive pattern.
Conclusion and recommendation: Weight loss and shortness of breath were significantly associated with culture positive Mycobacterium species. From this study, fungus was the most frequent infection identified in more than 50% of the study participants. Therefore, based on the findings of this study, we recommend applying multiple conventional culture techniques for isolation of bacterial and fungal agents before patients are registered as smear negative TB cases. It can help to identify the true etiological agents of the disease there by improving the diagnosis and treatment.
Jun Koh is doing his graduate course in Department of Surgery, College of Veterinary Medicine, Chonbuk National University from 2012. His major is small animal general surgery and a field of research is related in general surgery and hemorheology.
Whole blood viscosity which is major determinants of hemorheology is associated with cardiovascular, endocrinal, and cerebral nervous disease. An acquiring accurate blood viscosity measurement has been major issues in clinical pathology not even in veterinary medicine, and new type of scanning capillary tube viscometer proposes solution with continuous measurement of viscosity over whole range of shear rates. The objective of this study is to evaluate the normal range of whole blood viscosity and the correlation between whole blood viscosity and hematological data. The blood samples were collected from jugular vein by venipuncture in pigs (n=130). Whole blood viscosity measured within 4 hours after collection using scanning capillary tube viscometer. Scanning capillary tube viscometer which is capable of measuring yield stress and viscosity of whole blood continuously over a wide range of shear rates from 1s-1 to 1000s-1calculates viscosity using Casson fluid model. Mean diastolic and systolic blood viscosity of pigs were 34.66±7.84 and 4.88±0.85 respectively. Diastolic and systolic blood viscosity had a statistical significant correlation with the level of leukocyte (WBC), erythrocyte (RBC), hemoglobin (HGB), hematocrit (HCT), fibrinogen, alkaline phosphatase (ALP)and glucose in hematological examination (p<0.01). There were statistical significant differences of blood viscosity over whole range of shear rates among breeds and gender (p<0.05). The results suggested that correlation between whole blood viscosity and regular blood test such as complete blood cell count and serum chemistry in pigs and can be used as a reference data for further investigation.